Lipidated FRET-based reporter system that detects Neutrophil elastase (NE) activity.
|Appearance||1mM stock solution in DMSO|
|Purity||> 90% (HPLC-UV λ=443 nm)|
|Solubility||Soluble in DMSO|
|Size||10 µL / 50 µL|
|Storage||Keep cool and dry. Protect from light and moisture. Store at -20°C.|
NEmo is a FRET-based reporter system that detects Neutrophil elastase (NE) activity on cell surfaces or in solution. NE is a novel biomarker in patients with cystic fibrosis (CF) and potentially other chronic neutrophilic lung diseases.
Here you will find supporting information for NEmo application:
JoVE (DOI: 10.3791/62193-v)
Chung, J.; et al.: Increased Inflammatory Markers Detected in Nasal Lavage Correlate with Paranasal Sinus Abnormalities at MRI in Adolescent Patients with Cystic Fibrosis. Antioxidants 2021, 10,1412. https://doi.org/10.3390/antiox10091412
Margaroli et al.: Transcriptional firing represses bactericidal activity in cystic fibrosis airway neutrophils, 2021, Cell Reports Medicine 2, 100239 https://doi.org/10.1016/j.xcrm.2021.100239