caged-Ins(145)P3 / PM

<p><strong>Membrane-permeant derivatives of inositol phosphates </strong>require the intracellular enzymatic hydrolysis of several protecting groups in order to generate the biologically active compound.  The photolysis of membrane-permeant caged derivatives of Ins(145)P3 might mimic fast intracellular responses. In an initial step <strong>cells are loaded with the caged Ins(145)P3 derivative</strong>.</p> <p>Within 30 to 180 minutes all bioactivatable protecting groups remove generating <strong>caged inositol phosphate</strong>. The cage is known to prevent biological activity. The photochemical destruction of the cage (360-400nm) releases active Ins(145)P3 within a few seconds. Since this approach does not (directly) trigger other signaling events, membrane-permeant derivatives of signaling molecules are able to help dissecting <strong>signaling pathways</strong>.</p>